Identification and characterization of growth hormone receptors in snakehead fish (Ophiocephalus argus Cantor) liver
Publication in refereed journal

香港中文大學研究人員

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摘要The specific binding of I-125-labeled fish growth hormone (GH) to hepatic membranes prepared from several freshwater fish was assessed. A high level of growth hormone receptor (GHR) was detected on the hepatic membranes of the snakehead fish (Ophiocephalus argus Canter). Scatchard analysis of the binding data showed a single class of high affinity binding site with a binding affinity (K-a) of 1.45 +/- 0.23 x 10(9) M-1 and a binding capacity (B-max) of 198 +/- 57 fmol/mg protein. The binding was specific for fish GH and was saturable. In addition, the specific binding was temperature-and time-dependent, reaching a steady state after 16 hr of incubation at 25 degrees. The molecular weight of GHR as measured by Sephadex G-200 column chromatography and Western blot analysis using a monoclonal antibody (Mab263) against GHR was found to be 200-400 and 90-93 kDa, respectively. Two bands at 65 and 89 kDa were identified in ligand crosslinking studies of membrane receptors. A sensitive teleost GH radioreceptor assay (RRA) was developed, using recombinant fish GH and a membrane preparation from snakehead fish liver, capable of measuring bioactive GH in fish sera or other samples. (C) 1997 Academic Press.
著者Sun X, Zhu SQ, Chan SSH, Toresson G, Cheng CHK
期刊名稱General and Comparative Endocrinology
出版年份1997
月份12
日期1
卷號108
期次3
出版社ACADEMIC PRESS INC ELSEVIER SCIENCE
頁次374 - 385
國際標準期刊號0016-6480
電子國際標準期刊號1095-6840
語言英式英語
Web of Science 學科類別Endocrinology & Metabolism; ENDOCRINOLOGY & METABOLISM

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