Acute renal failure and proximal tubule lesions after trichosanthin injection in rats
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摘要The structural basis of the recently recognized renal impairment after infusion of trichosanthin (TCS). a type I ribosome inactivating protein, is uncertain, but functionally it appears to be related to a lesion in the renal tubules. In this study, renal dysfunction in experimental rats was induced by a single dose of TCS. Creatinine clearance and tubular proteinuria were used to assess renal function. Light microscopy and ultrastructure of the kidneys were examined and apoptosis in proximal tubules was evaluated by the ill situ TdT-mediated nick end labeling technique. TCS-treated rats demonstrated a significant dose-dependent decrease in creatinine clearance together with a mild degree of low-molecular-weight proteinuria. The proximal convoluted tubule was the site of lesions showing individual tubular cell death, which was more abundant in rats receiving high doses of TCS. Apoptotic cell death, together with heterophagosomes and large residual bodies, was observed. DNA fragmentation was confirmed by the in situ technique. There was also a dose-dependent density of apoptotic cells. Other portions of the nephron were spared, and it was not accompanied by any inflammatory infiltrate. In conclusion, these findings are consistent with TCS-induced proximal tubular toxicity resulting in reduction of glomerular filtration rate and tubular proteinuria. The extent of injury is dosage dependent. Both necrotic cell death and apoptosis participated in the loss of cells from the proximal tubules. Such toxicity may be mediated through intracellular events induced by trichosanthin. (C) 1997 Academic Press.
著者Tang NLS, Chan WL, Ke YB, Mak MKF, Lai FM, Tam SC
期刊名稱Experimental and Molecular Pathology
出版年份1997
月份4
日期1
卷號64
期次2
出版社ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
頁次78 - 89
國際標準期刊號0014-4800
語言英式英語
Web of Science 學科類別Pathology; PATHOLOGY

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