Acute renal failure and proximal tubule lesions after trichosanthin injection in rats
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AbstractThe structural basis of the recently recognized renal impairment after infusion of trichosanthin (TCS). a type I ribosome inactivating protein, is uncertain, but functionally it appears to be related to a lesion in the renal tubules. In this study, renal dysfunction in experimental rats was induced by a single dose of TCS. Creatinine clearance and tubular proteinuria were used to assess renal function. Light microscopy and ultrastructure of the kidneys were examined and apoptosis in proximal tubules was evaluated by the ill situ TdT-mediated nick end labeling technique. TCS-treated rats demonstrated a significant dose-dependent decrease in creatinine clearance together with a mild degree of low-molecular-weight proteinuria. The proximal convoluted tubule was the site of lesions showing individual tubular cell death, which was more abundant in rats receiving high doses of TCS. Apoptotic cell death, together with heterophagosomes and large residual bodies, was observed. DNA fragmentation was confirmed by the in situ technique. There was also a dose-dependent density of apoptotic cells. Other portions of the nephron were spared, and it was not accompanied by any inflammatory infiltrate. In conclusion, these findings are consistent with TCS-induced proximal tubular toxicity resulting in reduction of glomerular filtration rate and tubular proteinuria. The extent of injury is dosage dependent. Both necrotic cell death and apoptosis participated in the loss of cells from the proximal tubules. Such toxicity may be mediated through intracellular events induced by trichosanthin. (C) 1997 Academic Press.
All Author(s) ListTang NLS, Chan WL, Ke YB, Mak MKF, Lai FM, Tam SC
Journal nameExperimental and Molecular Pathology
Year1997
Month4
Day1
Volume Number64
Issue Number2
PublisherACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
Pages78 - 89
ISSN0014-4800
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesPathology; PATHOLOGY

Last updated on 2020-21-09 at 01:43