Construction of a human heart cDNA library and identification of cardiovascular based genes (CVBest)
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AbstractThe availability of high quality cDNA libraries is often crucial to the successful identification and characterization of genes. The concepts and potential pitfalls of constructing cDNA libraries are presented. Various applications requiring high quality cDNA libraries are outlined, including large-scale single pass sequencing of cDNA clones to generate expressed sequence tags (ESTs) and differential screening of cDNA libraries. The usefulness of combining such approaches for the discovery of novel disease-related and cardiovascular-based ESTs (CVBest) is discussed.
All Author(s) ListLiew CC, Hwang DM, Wang RX, Ng SH, Dempsey A, Wen DHY, Ma H, Cukerman E, Zhao XG, Liu YQ, Qiu XK, Zhou XM, Gu JR, Tsui S, Fung KP, Waye MMW, Lee CY
Journal nameMolecular and Cellular Biochemistry
Volume Number172
Issue Number1-2
Pages81 - 87
LanguagesEnglish-United Kingdom
KeywordsDNA; gene expression; heart; library
Web of Science Subject CategoriesCell Biology; CELL BIOLOGY

Last updated on 2020-12-10 at 01:09