Immunohistochemical localization of type-II (AT(2)) angiotensin receptors with a polyclonal antibody against a peptide from the C-terminal tail
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AbstractA polyclonal antibody has been prepared against a synthetic peptide derived from the C-terminal tail of the cloned rat AT(2) angiotensin receptor, corresponding to amino acid residue 341-351. The antibody was of high titer and displayed monospecific activity coward the synthetic peptide in the ELISA assay. Western blot analysis indicated that the antiserum recognised only a single protein band with a mean apparent molecular mass of 75.4 kDa in the rat adrenals. Immunohistochemical studies with affinity purified antibody localised immunoreactive AT(2) angiotensin receptor in medulla cells of the adrenals. Immunoreactivity was also observed in pyramidal tract, but no specific immunoreactivity can be detected in regions of rat brain that are known to express AT(2) angiotensin receptors, including inferior olive, locus coeruleus and cerebellum. (C) 1997 Elsevier Science B.V.
All Author(s) ListYiu AKL, Wong PF, Yeung SY, Lam SM, Luk SKS, Cheung WT
Journal nameRegulatory Peptides
Volume Number70
Issue Number1
Pages15 - 21
LanguagesEnglish-United Kingdom
Keywordsangiotensin; AT(2) angiotensin receptors; immunohistochemistry; peptide-directed antibody; rat adrenals
Web of Science Subject CategoriesEndocrinology & Metabolism; ENDOCRINOLOGY & METABOLISM; Physiology; PHYSIOLOGY

Last updated on 2020-23-10 at 02:04