Differential effects of pinacidil, cromakalim, and NS 1619 on electrically evoked contractions in rat vas deferens
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AbstractAIM: To compare the inhibitory action of electrically evoked contractions of rat epididymal vas deferens by pinacidil (Pin), cromakalim (Cro), and NS 1619. METHODS: Monophasic contractions were evoked by electric field stimulation in rat isolated epididymal half of vas deferens. RESULTS: Newly developed ATP-sensitive K+ channel openers, Pin and Cro, concentration-dependently reduced the electrically evoked (0.3 Hz, 1 ms pulse duration, 60 V) contractions and glibenclamide but not charybdotoxin antagonized the inhibitory effects of both agents. Pin shifted the concentration-response curve for norepinephrine to the right with reducing the magnitude of the maximum contraction in a glibenclamid-sensitive fashion. The large-conductance Ca2+-activated K+ channel opener, NS 1619, inhibited the electrically evoked contractions in a concentration-dependent manner. Charybdotoxin (100 nmol.L-1) partially reduced the effect of NS 1619 but glibenclamide (10 mu mol.L-1) showed no effect. None of these 3 agents affected the basal tension. Both ATP-sensitive and Ca2+-activated K+ channels presented in vas deferens smooth muscles involved in regulation of muscle contractility.
All Author(s) ListHuang Y, Lau CW
Journal nameACTA PHARMACOLOGICA SINICA
Year1997
Month7
Day1
Volume Number18
Issue Number4
PublisherACTA PHARMACOLOGICA SINICA
Pages293 - 298
ISSN0253-9756
LanguagesEnglish-United Kingdom
Keywordscharybdotoxin; cromakalim; electric stimulation; glyburide; muscle contraction; NS 1619; pinacidil; potassium channels; vas deferens
Web of Science Subject CategoriesChemistry; Chemistry, Multidisciplinary; CHEMISTRY, MULTIDISCIPLINARY; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY

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