Angiotensin II receptors, AT(1) and AT(2) in the rat epididymis immunocytochemical and electrophysiological studies
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AbstractPrevious work from our laboratory has provided evidence for the presence of a tissue renin-angiotensin system in the rat epididymis. In the current investigation, the regional localization of angiotensin II receptors, type I (AT(1)) and type II (AT(2)) was studied immunocytochemically using specific anti-peptide antibodies against the second extracellular loops of AT(1) and AT(2) receptors, and pharmacologically using specific receptor antagonists in conjunction with the short-circuit current technique, The immunocytochemical results showed that AT(1) and AT(2) immunoreactivities were predominantly localized in the basal region of the epididymal epithelium. Electrophysiological studies using the short-circuit current technique demonstrated a stimulatory effect of basolaterally applied angiotensin II on the epididymal electrogenic ion transport. This effect was inhibitable by the addition of AT(1) antagonist, losartan but not by AT(2) antagonist, PD123177, indicating a functional role of AT(1) in epididymal electrolyte transport. The present finding suggests that angiotensin II receptors may play an important role in the regulation of epididymal function.
All Author(s) ListLeung PS, Chan HC, Fu LXM, Zhou WL, Wong PYD
Journal nameBiochimica et Biophysica Acta (BBA) - Molecular Cell Research
Volume Number1357
Issue Number1
Pages65 - 72
LanguagesEnglish-United Kingdom
Keywords(rat); angiotensin II; angiotensin II receptor; electrophysiology; epididymis; epithelium; immunocytochemistry
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Cell Biology; CELL BIOLOGY

Last updated on 2020-26-10 at 02:21