High-performance liquid chromatographic determination of atropine and atropine-like alkaloids in pharmaceutical preparations with indirect conductometric detection
Publication in refereed journal

香港中文大學研究人員
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摘要An HPLC method using indirect conductometric detection is proposed for the determination of atropine and atropine-like alkaloids in pharmaceutical preparations. It involves the use of an Ultrasphere 5 mu m CN analytical column (250 mmX4.6 mm) and a mobile phase of water-acetonitrile-tetrahydrofuran (67:30:3, v/v/v), containing 1 mM perchloric acid for all alkaloids except hyoscine butylbromide, where in the mobile phase the ratio of water to acetonitrile was 47:50. Derivatisation of the drugs is not required. The detector responses for all the alkaloids are similar. The calibration graphs exhibited wide linear concentration range of 0-500 mu g ml(-1) for a sample size of 20 mu l for all the analytes. The method has been applied to determine the drugs in a number of pharmaceutical preparations.
著者Lau OW, Mok CS
期刊名稱Journal of Chromatography A
出版年份1997
月份4
日期4
卷號766
期次1-2
出版社ELSEVIER SCIENCE BV
頁次270 - 276
國際標準期刊號0021-9673
電子國際標準期刊號1873-3778
語言英式英語
關鍵詞atropine; homatropine; hyoscine
Web of Science 學科類別Biochemical Research Methods; BIOCHEMICAL RESEARCH METHODS; Biochemistry & Molecular Biology; Chemistry; Chemistry, Analytical; CHEMISTRY, ANALYTICAL

上次更新時間 2021-03-12 於 23:54