Electrogenic ion transport in the mouse endometrium: Functional aspects of the cultured epithelium
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AbstractA primary culture of mouse endometrial epithelium grown on permeable supports was established and the electrogenic ion transport across the endometrial epithelium was studied using the short-circuit current (I-SC) technique. Enzymatically isolated mouse endometrial cells were immunostained with epithelial cells markers, cytokeratins, indicating an epithelial origin of the culture. Mouse endometrial epithelial cells grown on Millipore filters formed polarized monolayers with junctional complexes as revealed by light and electron microscopy. The cultured monolayers exhibited an average basal I-SC of 4.6 +/- 0.3 mu A/cm(2), transepithelial voltage of 2.7 +/- 0.2 mV and transepithelial resistance of 599 +/- 30 Omega cm(2). The basal current was reduced by 85% in Na+-free solution and 13% in Cl--free solution. The basal current could also be substantially (57.7%) blocked by an apical Na+ channel blocker, amiloride (10 mu M), suggesting that Na+ absorption largely contributed to the basal current. Apical addition of Cl- channel blocker, DPC (2 mM), also exhibited an inhibitory effect. 19.4%, on the basal I-SC, indicating minor involvement of Cl- secretion as compared to that of Na+ absorption. The cultured endometrial epithelium also responded to a number of secretagogues including adrenaline and forskolin with increases in the I-SC, which could involve substantial Cl- secretion. The present study has established a culture of mouse endometrial epithelium exhibiting predominantly Na+ absorption under unstimulated condition, and Cl- secretion in response to various secretagogues. This culture may be useful for studying various regulatory mechanisms of electrogenic ion transport across the endometrial epithelium.
All Author(s) ListChan HC, Liu CQ, Fong SK, Law SH, Leung PS, Leung PY, Fu WO, Chew SBC, Wong PYD
Journal nameBiochimica et Biophysica Acta (BBA) - Molecular Cell Research
Volume Number1356
Issue Number2
Pages140 - 148
LanguagesEnglish-United Kingdom
KeywordsCl- secretion; endometrium; epithelium; Na+ reabsorption
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Cell Biology; CELL BIOLOGY

Last updated on 2021-19-04 at 00:32