Effect of nutrient nitrogen and manganese on manganese peroxidase and laccase production by Pleurotus sajor-caju
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AbstractPleurotus sajor-caju, strain Pl-27, produces manganese-dependent peroxidase (MnP) and laccase, but not lignin peroxidase, when grown on a defined medium with glucose as sole carbon source. MnP activity was detected in fungal cultures supplemented with both high (26 mM-N) and low (2.6 mM-N) nutrient nitrogen although higher specific activity values were recorded under the latter conditions. Conversely, laccase production was not influenced by nutrient nitrogen levels under the growth conditions adopted. Both the titre and time of appearence of MnP were also affected by the concentration of Mn in the culture medium with highest enzyme levels recorded in cultures supplemented with 15 ppm Mn. Two MnP and five laccase isoforms were identified by FPLC and gel electrophoresis.
All Author(s) ListFu SY, Yu HS, Buswell JA
Journal nameFEMS Microbiology Letters
Volume Number147
Issue Number1
Pages133 - 137
LanguagesEnglish-United Kingdom
Keywordslaccase; lignin degradation; manganese peroxidase; Pleurotus sajor-caju
Web of Science Subject CategoriesMicrobiology; MICROBIOLOGY

Last updated on 2021-13-10 at 00:54