A globular, not a symmetric, form of acetylcholinesterase is expressed in chick motor neurons: Down-regulation toward maturity and after denervation
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AbstractIn vertebrate neuromuscular junctions, the postsynaptic specializations include the accumulation of acetylcholinesterase (AChE) at the synaptic basal lamina and the muscle fiber. Several lines of evidence indicate that the presynaptic motor neuron is able to synthesize and secrete AChE at the neuromuscular junctions. By using anti-AChE catalytic subunit, anti-butyrylcholinesterase (BuChE) catalytic subunit, and anti-AChE collagenous tail monoclonal antibodies, we demonstrated that the motor neurons of chick spinal cord expressed AChE in vivo and the predominant AChE was the globular form of the enzyme, Neither asymmetric AChE nor BuChE was detected in the motor neurons. The molecular mass of AChE catalytic subunit in the motor neuron was similar to 105 kDa, which was similar to that of the globular enzyme from low-salt extracts of muscle both of them were similar to 5 kDa smaller than the asymmetric AChE from high-salt extracts of muscle, The level of AChE expression in the motor neurons decreased, as found by immunochemical and enzymatic analysis, during the different stages of the chick's development and after nerve lesion. Thus, the AChE activity at the neuromuscular junctions that is contributed by the presynaptic motor neurons is primarily the globular, not the asymmetric, form of the enzyme, and these contributions decreased toward maturity and after denervation.
All Author(s) ListTsim KWK, Choi RCY, Dong TTX, Wan DCC
Journal nameJournal of Neurochemistry
Volume Number68
Issue Number2
Pages479 - 487
LanguagesEnglish-United Kingdom
Keywordsbutyrylcholinesterase; collagen-like tail; muscle development; neuromuscular junctions; postsynaptic specializations
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Neurosciences; NEUROSCIENCES; Neurosciences & Neurology

Last updated on 2020-15-09 at 00:45