Demonstration of ribonuclease activity in the plant ribosome-inactivating proteins alpha- and beta-momorcharins
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摘要Alpha- and beta-momorcharins, ribosome-inactivating proteins from Momordica charantia seeds, were utilized in this investigation. Ribonucleolytic cleavage was observed after naked rRNA was incubated with either momorcharin. Beta-momorcharin, and to a lesser extent alpha-momorcharin, also acted on tRNA to release acid-soluble UV-absorbing products. Such activity was optimal at pH around 5.5. Using polyhomoribonucleotides as substrate, it was found that the momorcharins preferentially acted on polyU, but exerted negligible effects on polyA, polyC and polyG. Chromatographic analysis of the reaction product indicated that mono and/or oligo-ribonucleotides, but not free base, were generated from polyU, suggesting that the enzymatic action involved ribonucleolytic cleavage. Similar to the results obtained with tRNA as substrate, beta-momorcharin was about 15-fold more active than alpha-momorcharin on polyU.
著者Mock JWY, Ng TB, Wong RNS, Yao QZ, Yeung HW, Fong WP
期刊名稱Life Sciences
出版年份1996
月份10
日期25
卷號59
期次22
出版社PERGAMON-ELSEVIER SCIENCE LTD
頁次1853 - 1859
國際標準期刊號0024-3205
電子國際標準期刊號1879-0631
語言英式英語
關鍵詞Momordica charantia; ribonuclease; ribosome-inactivation proteins
Web of Science 學科類別Medicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY; Research & Experimental Medicine

上次更新時間 2020-17-10 於 02:27