Identification and characterization of various differentiative growth plate chondrocytes from porcine by countercurrent centrifugal elutriation
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AbstractCountercurrent centrifugal elutriation was used to separate growth plate chondrocytes from porcine basing on their differences in sizes and densities. Eighteen fractions of cells with different sizes and densities were obtained. The mean cellular volumes increased progressively in each of successive fractions, and that increase was associated with specific phenotypic changes, such as biochemical differences in DNA synthesis, proteoglycan synthesis, and activities of alkaline phosphatase. Three distinct chondrocyte subpopulations with their unique characteristics were identified among the elutriated fractions. The resting chondrocytes were found to be small in size and quiescent. The hypertrophic chondrocytes were found to be large in size and metabolically active both in alkaline phosphatase and in proteoglycan productions. The proliferative chondrocytes exhibited a high DNA synthesis rate, and their sizes were found to be between those of the resting and hypertrophic chondrocytes. (C) 1996 Wiley-Liss, Inc.
All Author(s) ListLee KM, Fung KP, Leung PC, Leung KS
Journal nameJournal of Cellular Biochemistry
Year1996
Month3
Day15
Volume Number60
Issue Number4
PublisherWILEY-LISS
Pages508 - 520
ISSN0730-2312
eISSN1097-4644
LanguagesEnglish-United Kingdom
Keywordsalkaline phosphatase; cartilage; chondrocyte; countercurrent centrifugal elutriation; porcine
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Cell Biology; CELL BIOLOGY

Last updated on 2020-17-10 at 02:21