Use of trichosanthin to reduce infection by turnip mosaic virus
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摘要Exogenous application of recombinant trichosanthin (TCS) inhibited local lesion formation by turnip mosaic virus (TuMV) in leaves of Nicotiana tabacum and caused a delay in the development of mosaic symptoms by TuMV in Brassica parachinensis. A dose-response relationship was observed in the latter case. To produce transgenic plants expressing a TCS cDNA, a chimeric gene which contains a fusion between the cauliflower mosaic virus 35S promoter, the TCS-encoding cDNA and the octopine synthase 3' polyadenylation sequence (ocs 3') was constructed. The chimeric gene was then cloned into a binary vector and introduced into the genome of N. tabacum cv. 'Wisconsin 38' via Agrobacterium tumefaciens-mediated transformation. Three transgenic tobacco plants which synthesized TCS protein in the cytosol were completely resistant to mechanical inoculation of TuMV.
著者Lam YH, Wong YS, Wang B, Wong RNS, Yeung HW, Shaw PC
期刊名稱Plant Science
出版年份1996
月份2
日期9
卷號114
期次1
出版社ELSEVIER SCI IRELAND LTD
頁次111 - 117
國際標準期刊號0168-9452
語言英式英語
關鍵詞anti-viral assays; Brassica parachinensis; Nicotiana tabacum; ribosome inactivating protein; trichosanthin; turnip mosaic virus
Web of Science 學科類別Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Plant Sciences; PLANT SCIENCES

上次更新時間 2020-17-10 於 02:17