EFFECT OF HYPERTHERMIA ON INTRACELLULAR PH IN HUMAN U-87 MG GLIOBLASTOMA CELLS
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摘要The effect of hyperthermia at 43 degrees C on intracellular pH (pH(i)) in human U-87 MG glioblastoma cells was studied by using the fluorescent probe 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein-pentaacetoxymethyl ester. The presence of Na+/H+ antiporter activity in the cells were demonstrated by the Na+-dependent increase in intracellular pH (pH(i)) after cellular acidification in the absence of HCO3-. Hyperthermia at 43 degrees C caused significant decrease in pH(i). The acidification was readily reversible by cooling the cells back down to 37 degrees C. The pH(i) change was inhibited by the addition of 1 mM amiloride in the incubation medium. Amiloride and hyperthermia exhibited a synergistic effect in suppressing thymidine incorporation into the cells.
著者LUI CP, FUNG KP, KONG SK, CHOY YM, LEE CY
期刊名稱Oncology
出版年份1995
月份11
日期1
卷號52
期次6
出版社KARGER
頁次492 - 497
國際標準期刊號0030-2414
語言英式英語
關鍵詞HUMAN GLIOBLASTOMA CELL; HYPERTHERMIA; INTRACELLULAR PH
Web of Science 學科類別Oncology; ONCOLOGY

上次更新時間 2020-31-07 於 01:27