A BACTERIAL METHYLTRANSFERASE M.ECOHK31I REQUIRES 2 PROTEINS FOR IN-VITRO METHYLATION
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AbstractThe genes encoding EcoHK31l restriction-modification (R-M) system were isolated from a clinically-isolated Escherichia coli strain HK31. The entire R-M system of EcoHK31l is located in a 2.1 kb fragment. R.EcoHK31l is an isoschizomer of Eael which recognizes and cleaves Y down arrow GGCCR. M.EcoHK31l consists of two polypeptides alpha and beta with sites 309 and 176 aa, respectively. Polypeptide beta is encoded within an alternative reading frame of polypeptide alpha. All the conserved motifs in mC5-MTases can be found in polypeptide alpha except motif IX which is present in polypeptide beta. Polypeptides alpha and beta were separately synthesized in a T7 promoter controlled over-expression system and in vitro methylation occurred only when the two extracts were mixed and thus confirms that two polypeptides are required for methylation.
All Author(s) ListLEE KF, KAM KM, SHAW PC
Journal nameNucleic Acids Research
Year1995
Month1
Day11
Volume Number23
Issue Number1
PublisherOXFORD UNIV PRESS
Pages103 - 108
ISSN0305-1048
eISSN1362-4962
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY

Last updated on 2020-31-05 at 02:56