ISOLATION AND CHARACTERIZATION OF A CDNA THAT CODES FOR A LIM-CONTAINING PROTEIN WHICH IS DEVELOPMENTALLY-REGULATED IN HEART
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AbstractDuring our human heart cDNA sequencing project, we have obtained a novel cDNA clone which is very similar in DNA and amino acid sequences to a rat/mouse cysteine-rich intestinal protein (1). Sequence analysis has shown that this human cysteine-rich heart protein (hCRHP) is a protein of 77 amino acids and possesses a LIM motif which is considered to be able to bind zinc. Northern blot analyses have shown that its mRNA level in rat heart is regulated developmentally. We have expressed hCRHP in E. coli using pAED4 as the vector and the cDNA was engineered so that the authentic protein is produced. The protein was partially purified and was shown to be a basic protein. (C) 1994 Academic Press, Inc.
All Author(s) ListTSUI SKW, YAM NYH, LEE CY, WAYE MMY
Journal nameBiochemical and Biophysical Research Communications
Year1994
Month11
Day30
Volume Number205
Issue Number1
PublisherACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
Pages497 - 505
ISSN0006-291X
eISSN1090-2104
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biophysics; BIOPHYSICS

Last updated on 2021-25-02 at 02:22