THE INDUCTION OF CYTOKINE GENE-EXPRESSION IN MURINE PERITONEAL-MACROPHAGES BY PSEUDOSTELLARIA-HETEROPHYLLA
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AbstractWe have previously shown that a mitogenic fraction (PH-I) separated from Pseudostellaria heterophylla (P. heterophylla) could act as a priming agent for the release of tumor necrosis factor-alpha (TNF-alpha) in mice. In the present study, PH-I was further purified by gel filtration chromatography and the resulting three fractions (PH-I A, PH-I B and PH-I C) were assessed for the induction of TNF-alpha, interleukin-1-alpha (IL-1-alpha) and IL-1-beta gene expression in mice by enzyme-linked immunosorbent assay and polymerase chain reaction. It was found that fraction PH-I C from P. heterophylla was the most potent priming fraction among the three fractions for the induction of TNF-alpha in serum and the TNF-alpha mRNA in murine macrophages. Moreover, all three fractions were found to increase the expression of IL-1-alpha mRNA while PH-I 61 showed the most potent activating effect on the expression of IL-1-beta mRNA.
All Author(s) ListWONG CK, LEUNG KN, FUNG MC, FUNG KP, CHOY YM
Journal nameImmunopharmacology and Immunotoxicology
Year1994
Month1
Day1
Volume Number16
Issue Number3
PublisherMARCEL DEKKER INC
Pages347 - 357
ISSN0892-3973
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesImmunology; IMMUNOLOGY; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY; Toxicology; TOXICOLOGY

Last updated on 2020-15-07 at 01:13