THE NITRIC-OXIDE DONOR, SODIUM-NITROPRUSSIDE, INCREASED INTRANUCLEAR AND CYTOSOLIC-FREE CALCIUM-CONCENTRATION IN SINGLE PU5-1.8 CELLS
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摘要In the present study, the effect of sodium nitroprusside (SNP), a nitric oxide (NO) releasing agent, on the intracelluar free calcium concentration ([Ca2+]i) was studied. Our results demonstrate that SNP could increase [Ca2+]i in single PU5-1.8 cells, a murine macrophage cell line, as determined by confocal laser scanning microscope with calcium green-1 fluorescent probe. In the presence of hemoglobin, a NO scavenger, the effect of SNP on the Ca2+ level was suppressed. Our data also show that the intranuclear Ca2+ ([Ca2+]n) was always higher than those in cytosol. Stimulation of cells with 1mM SNP induced an immediate increase of [Ca2+]n with a delayed rise of cytosolic Ca2+ ([Ca2+]c). Interestingly, when cells were subsequently treated with 2mM SNP, an increase of [Ca2+]c with a similar pattern to the rise [Ca2+]n was observed. In Ca2+-free buffer, SNP also raised the [Ca2+]n and [Ca2+]c. When Ca2+ was added to the buffer, a second peak of [Ca2+]n and several spikes of [Ca2+]c were observed. These results thus indicate that 1) NO may increase both [Ca2+]n and [Ca2+]c in PU5-1.8 cells; 2) the increase of [Ca2+]n requires both internal and external Ca2+. Moreover, the diverse responses in these cells suggest that the regulation of Ca2+ in these areas may be different. (C) 1994 Academic Press, Inc.
著者KONG SK, CHOY YM, LEE CY
期刊名稱Biochemical and Biophysical Research Communications
出版年份1994
月份2
日期28
卷號199
期次1
出版社ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
頁次234 - 240
國際標準期刊號0006-291X
電子國際標準期刊號1090-2104
語言英式英語
Web of Science 學科類別Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biophysics; BIOPHYSICS

上次更新時間 2020-07-08 於 01:33