THE NITRIC-OXIDE DONOR, SODIUM-NITROPRUSSIDE, INCREASED INTRANUCLEAR AND CYTOSOLIC-FREE CALCIUM-CONCENTRATION IN SINGLE PU5-1.8 CELLS
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AbstractIn the present study, the effect of sodium nitroprusside (SNP), a nitric oxide (NO) releasing agent, on the intracelluar free calcium concentration ([Ca2+]i) was studied. Our results demonstrate that SNP could increase [Ca2+]i in single PU5-1.8 cells, a murine macrophage cell line, as determined by confocal laser scanning microscope with calcium green-1 fluorescent probe. In the presence of hemoglobin, a NO scavenger, the effect of SNP on the Ca2+ level was suppressed. Our data also show that the intranuclear Ca2+ ([Ca2+]n) was always higher than those in cytosol. Stimulation of cells with 1mM SNP induced an immediate increase of [Ca2+]n with a delayed rise of cytosolic Ca2+ ([Ca2+]c). Interestingly, when cells were subsequently treated with 2mM SNP, an increase of [Ca2+]c with a similar pattern to the rise [Ca2+]n was observed. In Ca2+-free buffer, SNP also raised the [Ca2+]n and [Ca2+]c. When Ca2+ was added to the buffer, a second peak of [Ca2+]n and several spikes of [Ca2+]c were observed. These results thus indicate that 1) NO may increase both [Ca2+]n and [Ca2+]c in PU5-1.8 cells; 2) the increase of [Ca2+]n requires both internal and external Ca2+. Moreover, the diverse responses in these cells suggest that the regulation of Ca2+ in these areas may be different. (C) 1994 Academic Press, Inc.
All Author(s) ListKONG SK, CHOY YM, LEE CY
Journal nameBiochemical and Biophysical Research Communications
Year1994
Month2
Day28
Volume Number199
Issue Number1
PublisherACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
Pages234 - 240
ISSN0006-291X
eISSN1090-2104
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biophysics; BIOPHYSICS

Last updated on 2020-06-07 at 01:18