RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM OF HLA-DQ AND HLA-DR ALLOGENOTYPES IN NORMAL SOUTHERN CHINESE
Publication in refereed journal


全文

引用次數

其它資訊
摘要We have examined the Tag I restriction fragment length polymorphism (RFLP) of the MHC class II regions of 96 normal Southern Chinese with respect to the HLA-DRB, -DQA, and -DQB genes. The data were compared with the DR and DQ serotyping. RFLP correlates well with the serological typing of the DR types of Chinese. Variants are seen in DR2 and DR12. The variant in DR12 with a 10 kb at the upper band (DR12b) is not found in Caucasoids and is more frequent in Chinese (19.4% of the alleles). With the help of DQA and DQB RFLP the assignment of genotyping of DRB types is facilitated. Some of the linkage disequilibrium patterns among DRB, DQA and DQB are different from Caucasoids. This is particularly obvious in the DR2, and DR5 haplotypes. In the 96 Southern Chinese, the 3 commonest haplotype frequencies are: DR12b, DQ alpha 1b, DQ beta 3b (18.3%); DR9, DQ alpha 3, DQ beta 3a (15.7%); DR4, DQ alpha 3, DQ beta 3a (14.2%). The various DR beta, DQ alpha. DQ beta genes can be accurately defined in Chinese by RFLP. Polymerase chain reactions using sequence-specific primers were performed to confirm the various HLA-DRB and -DQB alleles. The use of RFLP is important in the study of HLA matching and HLA-disease association in Southern Chinese.
著者LI PKT, POON ASY, HAWKINS BR, YEUNG VTF, BO YH, LAI KN
期刊名稱Tissue Antigens
出版年份1993
月份11
日期1
卷號42
期次5
出版社MUNKSGAARD INT PUBL LTD
頁次502 - 508
國際標準期刊號0001-2815
語言英式英語
關鍵詞DQ; DR; HLA; RFLP; SOUTHERN CHINESE
Web of Science 學科類別Cell Biology; CELL BIOLOGY; Immunology; IMMUNOLOGY; Pathology; PATHOLOGY

上次更新時間 2021-02-03 於 01:48