EFFECTS OF HYPERTHERMIA ON THE NUCLEOLAR PROTEINS IN TUMOR-CELLS
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AbstractThe effects of hyperthermia at 41-degrees-C and 43-degrees-C on the nucleolar protein B23 in Ehrlich ascites tumour (EAT), human glioblastoma U-87 MG and U-373 MG cell lines were studied. Cellular localization of protein B23 was detected by an immunofluorescence technique using monoclonal antibody against protein B23. Diminution of fluorescence in the nucleoli occurred when the cells were treated at high temperature. The decrease in fluorescence level depends on the treatment temperature and duration. Among the three cell lines studied, the U-373 MG glioblastoma was the least responsive to hyperthermia followed by the U-87 MG glioblastoma. The decrease in nucleolar fluorescence of the EAT cells treated at 41-degrees-C and 43-degrees-C correlated with their subsequent cell survival. Dispersion of the nucleolar argyrophilic granules occurred in EAT cells after heating at 43-degrees-C for 1 h. The possible implication of such effect is discussed in relation to the heat-sensitive elements in the nucleolus.
All Author(s) ListLUI CP, CHAN PK, FUNG KP, CHOY YM, LEE CY
Journal nameCancer Letters
Year1993
Month6
Day15
Volume Number70
Issue Number1-2
PublisherELSEVIER SCI IRELAND LTD
Pages129 - 139
ISSN0304-3835
eISSN1872-7980
LanguagesEnglish-United Kingdom
KeywordsHYPERTHERMIA; NUCLEOLAR PROTEINS; PROTEIN-B23; SILVER STAINING
Web of Science Subject CategoriesOncology; ONCOLOGY

Last updated on 2020-08-07 at 05:13