ANALYSIS OF CHARGE OF KAPPA AND LAMBDA CHAIN-CONTAINING SERUM IMMUNOGLOBULIN-A BY IMMUNOBLOTTING AND DENSITOMETRY
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摘要A novel method for studying the charge distribution of different subclasses of serum immunoglobulin A (defined by the light chains) is described. Affinity-chromatography purified immunoglobulins were focused in polyacrylamide gel and were then transferred electrophoretically onto nitrocellulose membranes. The transferred immunoglobulins were detected by rabbit antiserum to human kappa (kappa) or lambda (lambda) chain, swine anti-rabbit IgG, rabbit anti-peroxidase antibodies and peroxidase, together with a substrate solution comprising H2O2 and diaminobenzidine. Finally, the developed membranes were made transparent with Triton-x 114, scanned at 485 nm with a densitometer to obtain quantitation of charge distribution.
著者CHUI SH, LEWIS WHP, LAM CWK, LAI KN
期刊名稱Clinical Biochemistry
出版年份1993
月份4
日期1
卷號26
期次2
出版社PERGAMON-ELSEVIER SCIENCE LTD
頁次97 - 100
國際標準期刊號0009-9120
電子國際標準期刊號1873-2933
語言英式英語
關鍵詞IMMUNOBLOTTING; IMMUNOGLOBULIN CHARGE; KAPPA IGA; LAMBDA IGA
Web of Science 學科類別Medical Laboratory Technology; MEDICAL LABORATORY TECHNOLOGY

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