ABSENCE OF P53 GENE-MUTATIONS IN PRIMARY NASOPHARYNGEAL CARCINOMAS
Publication in refereed journal

香港中文大學研究人員

全文

引用次數

其它資訊
摘要Alterations in the p53 tumor suppressor gene and Epstein-Barr virus status were investigated in 15 nasopharyngeal carcinoma (NPC) biopsies. 4 xenografts, and 2 cell lines from the Cantonese region of southern China. One other established NPC cell line obtained from a northern Chinese patient was also studied. Restriction fragment length polymorphism analysis revealed a loss of heterozygosity for chromosome 17p, where the p53 gene resides, in only one of 15 NPC biopsies. Polymerase chain reaction-single-stranded conformational polymorphism analysis and direct sequencing failed to detect sequence alterations in exons 5 through 8 of the p53 gene in the 15 tumors and in the 4 NPC xenografts, all of which tested positive for Epstein-Barr virus. In contrast, the 3 NPC cell lines were all negative for Epstein-Barr virus and contained G --> C transversions in the p53 gene, with cell lines CNE-1 and CNE-2 harboring identical AGA (arginine) to ACA (threonine) changes at codon 280. These results suggest that p53 inactivation is not a necessary component of nasopharyngeal carcinogenesis in Cantonese but may be important in the establishment of cell lines derived from these tumors.
著者SPRUCK CH, TSAI YC, HUANG DP, YANG AS, RIDEOUT WM, GONZALEZZULUETA M, CHOI P, LO KW, YU MC, JONES PA
期刊名稱Cancer Research
出版年份1992
月份9
日期1
卷號52
期次17
出版社AMER ASSOC CANCER RESEARCH
頁次4787 - 4790
國際標準期刊號0008-5472
電子國際標準期刊號1538-7445
語言英式英語
Web of Science 學科類別Oncology; ONCOLOGY

上次更新時間 2021-12-01 於 00:49