ABSENCE OF P53 GENE-MUTATIONS IN PRIMARY NASOPHARYNGEAL CARCINOMAS
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AbstractAlterations in the p53 tumor suppressor gene and Epstein-Barr virus status were investigated in 15 nasopharyngeal carcinoma (NPC) biopsies. 4 xenografts, and 2 cell lines from the Cantonese region of southern China. One other established NPC cell line obtained from a northern Chinese patient was also studied. Restriction fragment length polymorphism analysis revealed a loss of heterozygosity for chromosome 17p, where the p53 gene resides, in only one of 15 NPC biopsies. Polymerase chain reaction-single-stranded conformational polymorphism analysis and direct sequencing failed to detect sequence alterations in exons 5 through 8 of the p53 gene in the 15 tumors and in the 4 NPC xenografts, all of which tested positive for Epstein-Barr virus. In contrast, the 3 NPC cell lines were all negative for Epstein-Barr virus and contained G --> C transversions in the p53 gene, with cell lines CNE-1 and CNE-2 harboring identical AGA (arginine) to ACA (threonine) changes at codon 280. These results suggest that p53 inactivation is not a necessary component of nasopharyngeal carcinogenesis in Cantonese but may be important in the establishment of cell lines derived from these tumors.
All Author(s) ListSPRUCK CH, TSAI YC, HUANG DP, YANG AS, RIDEOUT WM, GONZALEZZULUETA M, CHOI P, LO KW, YU MC, JONES PA
Journal nameCancer Research
Year1992
Month9
Day1
Volume Number52
Issue Number17
PublisherAMER ASSOC CANCER RESEARCH
Pages4787 - 4790
ISSN0008-5472
eISSN1538-7445
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesOncology; ONCOLOGY

Last updated on 2021-18-05 at 01:21