ACTIVATION OF PROTEIN-KINASE-C ATTENUATES THE CYCLIC-GMP RESPONSES TO C-TYPE NATRIURETIC PEPTIDE IN CULTURED MOUSE ASTROCYTES
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AbstractC-type natriuretic peptide (CNP), a recently discovered natriuretic peptide, has a potent stimulatory effect on cyclic GMP (cGMP) formation in cultured mouse astrocytes. Pretreatment of astrocytes with phorbol 12-myristate 13-acetate (PMA), an activator of protein kinase C (PKC), attenuated CNP-induced cGMP responses in a dose-dependent manner, with a half-maximal inhibitory concentration of 6 nM, whereas the inactive phorbol ester analog, 4-alpha-phorbol 12,13-didecanoate, was without effect. In the presence of staurosporine, a PKC inhibitor, the inhibitory effect of PMA on CNP-stimulated cGMP production was reversed. These results suggest that PKC is an inhibitory modulator of CNP-stimulated cGMP responses in astrocytes and that CNP may interact with neuropeptides which stimulate PKC.
All Author(s) ListYEUNG VTF, HO SKS, COCKRAM CS, LEE CM, NICHOLLS MG
Journal nameFEBS Letters
Year1992
Month8
Day24
Volume Number308
Issue Number3
PublisherELSEVIER SCIENCE BV
Pages301 - 304
ISSN0014-5793
eISSN1873-3468
LanguagesEnglish-United Kingdom
KeywordsASTROCYTE; C-TYPE NATRIURETIC PEPTIDE; CYCLIC GMP; PROTEIN KINASE-C
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biophysics; BIOPHYSICS; Cell Biology; CELL BIOLOGY

Last updated on 2020-28-11 at 01:15