THE CONTRIBUTION OF STEROIDS TO DIGOXIN-LIKE IMMUNOREACTIVITY IN CORD BLOOD
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AbstractSamples of cord serum from 29 healthy neonates were analysed for digoxin-like immunoreactive substance (DLIS), cortisol, 17-beta-oestradiol, progesterone, dehydroepiandrosterone-sulphate (DHEAS), 17-alpha-hydroxyprogesterone (17OHP), androstenedione, oestriol and ouabain-like activity (OLA; by inhibition of Na+,K+ATPase activity). The mean serum concentration of DLIS was 0.91 (SD = 0.19) nmol/L and the mean OLA was 26.1 (SD = 11.5) nmol/L. There was no correlation between DLIS and OLA. DLIS correlated significantly with oestriol (r = 0.521), progesterone (r = 0.534) and 17OHP (r = 0.43). Stepwise multiple regression analysis showed that 17-beta-oestradiol, progesterone and androstenedione contributed to DL IS and the intercept was 0.64 (SD = 0.127). The concentrations of steroids (I7-beta-oestradiol, progesterone, androstenedione) required to displace digoxin by 50% in the digoxin immunoassay and inhibit Na+,K+ATPase in the OLA assay were 10(3)-10(4)-fold higher than those found in cord serum. We conclude that the contribution of these steroids to DLIS is small and that DLIS and OLA measure different compounds.
All Author(s) ListWONG SY, HO CS, PANESAR NS, SWAMINATHAN R
Journal nameAnnals of Clinical Biochemistry
Year1992
Month5
Day1
Volume Number29
PublisherROYAL SOC MEDICINE SERVICES LTD
Pages337 - 342
ISSN0004-5632
eISSN1758-1001
LanguagesEnglish-United Kingdom
Keywords17-HYDROXYPROGESTERONE PROGESTERONE; DIGOXIN; NA+,K+ATPASE INHIBITOR
Web of Science Subject CategoriesMedical Laboratory Technology; MEDICAL LABORATORY TECHNOLOGY

Last updated on 2020-10-07 at 05:10