LIGHT CHAIN RATIOS OF SERUM IMMUNOGLOBULINS IN DISEASE
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AbstractWe have determined the individual kappa (kappa)/ lambda (lambda) ratios of serum IgG, IgA, and IgM in normal subjects and patients with rheumatoid arthritis, systemic lupus erythematosus, hepatic cirrhosis and IgA nephropathy - 40 in each group. Serum samples were first screened by agarose electrophoresis to exclude paraproteinaemia. Concentrations of IgG, IgA, and IgM were determined by enzyme-linked immunosorbent assay (ELISA). The kappa and lambda-chain concentrations of each immunoglobulin class were assayed by an ELISA method first developed by us for the determination of kappa/lambda ratios. Our results showed that kappa/lambda ratios of serum IgA and IgM were significantly different from that of IgG in normal subjects and the 4 groups of patients studied (p < 0.01). The kappa/lambda ratios of individual immunoglobulins in patients with rheumatoid arthritis, systemic lupus erythematosus and liver cirrhosis were similar to those of normal subjects. However, patients with IgA nephropathy displayed a distinctly lower IgA kappa/lambda ratio, suggesting a unique antibody response in the immunopathogenesis of this disease.
All Author(s) ListLAM CWK, CHUI SH, LEUNG NWY, LI EKM, LAI KN
Journal nameClinical Biochemistry
Year1991
Month6
Day1
Volume Number24
Issue Number3
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Pages283 - 287
ISSN0009-9120
eISSN1873-2933
LanguagesEnglish-United Kingdom
KeywordsELISA; IGA NEPHROPATHY; IMMUNE-COMPLEX DISEASE; LIGHT CHAIN RATIOS; SERUM IMMUNOGLOBULINS
Web of Science Subject CategoriesMedical Laboratory Technology; MEDICAL LABORATORY TECHNOLOGY

Last updated on 2020-28-11 at 01:12