Generation of monoclonal antibodies against Hong Kong nasopharyngeal carcinoma-associated Epstein-Barr virus latent membrane protein 1 (LMP1)
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摘要A panel of monoclonal antibodies specific to Hong Kong Chinese nasopharyngeal carcinoma (NPC)-associated Epstein-Barr virus (EBV) latent membrane protein 1 (LMP!) variants has been generated. These monoclonal antibodies not only differentiate the Hong Kong Chinese NPC-associated LMP! variants from the prototype B95-8 LMP!, derived from Caucasian infectious mononucleosis, but also differentiate the 2 highly homologous LMP! deletion variants commonly found in Hong Kong primary NPC. The predominant deletion type variant, DV-Asp335, is characterized by an aspartic acid at residue 335 located in the cytoplasmic C-terminal region, whereas the other minor deletion variant, DV-Gly335, has a glycine in the same residue position. 335D is hitherto found predominantly in LMP! of the China ! strain in association with NPC in the Chinese populations located in southern China and Malaysia. These antibodies, which are applicable in ELISA, immunofluorescence, immunoprecipitation, immunoblotting and immunohistochemistry on paraffin sections, are the first variant-specific anti-LMP! monoclonal antibodies produced, and will be useful in investigating the functional significance of 335D in NPC. (C) 2002 Wiley-Liss, Inc.
著者Chan BCL, To KF, Pang JCS, Chung YF, Lo KW, Tong JHM, Huang DWS, Lim PL, Chui YL
期刊名稱International Journal of Cancer
出版年份2002
月份12
日期10
卷號102
期次5
出版社WILEY-LISS
頁次492 - 498
國際標準期刊號0020-7136
電子國際標準期刊號1097-0215
語言英式英語
關鍵詞EBV LMP1 variant-specific monoclonal antibodies; nasopharyngeal carcinoma
Web of Science 學科類別Oncology; ONCOLOGY

上次更新時間 2020-21-11 於 01:22