Anti-HIV-1 property of trichosanthin correlates with its ribosome inactivating activity
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AbstractTrichosanthin (TCS) is a type I ribosome inactivating (RI) protein possessing anti-tumor and antiviral activity, including human immunodeficiency virus (HIV). The mechanism of these actions is not entirely clear, but is generally attributed to its RI property. In order to study the relationship between the anti-HIV-1 activity of TCS and its RI activity, three TCS mutants with different RI activities were constructed by using site-directed mutagenesis. The anti-HIV-1 activities of the three mutants were tested in vitro. Results showed that two TCS mutants, namely TCSM((120-123)), TCSE160A/E189A, with the greatest decrease in RI activity, lost almost all of the anti-HIV activity and cytopathic effect. Another mutant TCSR122G, which exhibited a 160-fold decrease in RI activity, retained some anti-HIV activity. The results from this study suggested that RI activity of TCS may have significant contribution to its anti-HIV-1 property. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
All Author(s) ListWang JH, Nie HL, Tam SC, Huang H, Zheng YT
Journal nameFEBS Letters
Year2002
Month11
Day6
Volume Number531
Issue Number2
PublisherELSEVIER SCIENCE BV
Pages295 - 298
ISSN0014-5793
eISSN1873-3468
LanguagesEnglish-United Kingdom
Keywordsantiviral activity; human immunodeficiency virus type 1; ribosome inactivating protein; trichosanthin
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biophysics; BIOPHYSICS; Cell Biology; CELL BIOLOGY

Last updated on 2020-28-10 at 01:38