A locally generated angiotensin system in rat carotid body
Publication in refereed journal

香港中文大學研究人員

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摘要Previous studies have shown the existence of functional angiotensin II receptors in rat carotid body, which directly alters the carotid chemoreceptor afferent nerve activity. Moreover, chronic hypoxia could result in an enhanced sensitivity of chemoreceptor afferent activity via an AT(1) receptor-mediated calcium signaling in the carotid body. In the present study, the localization and expression of angiotensinogen, the obligatory component for an intrinsic, angiotensin-generating system, were investigated by in situ hybridization histochemistry, immunohistochemistry, RT-PCR, Western blot and Northern blot analysis. In situ hybridization showed the expression of angiotensinogen within the glomus cells of the carotid body. Double immunostaining of angiotensinogen and tyrosine hydroxylase, an immunohistochemical marker for type I glomus cells, elucidated that angiotensinogen protein was specifically localized to the lobules of type I cells. Consistently, RT-PCR and Western blot analysis confirmed the expression of angiotensinogen mRNA and protein, respectively. On the other hand, renin mRNA was not detected using RT-PCR and Northern blot analysis whereas angiotensin-converting enzyme (ACE) mRNA was detected in the carotid body. These data suggest that a locally generated angiotensin system is operated in the carotid body, which might be linked to a renin-independent biosynthetic pathway. Such an intrinsic, angiotensin-generating system and its local regulation by chronic hypoxia should be important in the modulation of cardiopulmonary adaptation in the hypoxic ventilatory response and the electrolyte as well as water homeostasis. (C) 2002 Elsevier Science B.V. All rights reserved.
著者Lam SY, Leung PS
期刊名稱Regulatory Peptides
出版年份2002
月份7
日期15
卷號107
期次1-3
出版社ELSEVIER SCIENCE BV
頁次97 - 103
國際標準期刊號0167-0115
電子國際標準期刊號1873-1686
語言英式英語
關鍵詞angiotensinogen; carotid body; renin-angiotensin system; type I glomus cells
Web of Science 學科類別Endocrinology & Metabolism; ENDOCRINOLOGY & METABOLISM; Physiology; PHYSIOLOGY

上次更新時間 2020-26-10 於 00:41