The extraction of imperialine and imperialine-3 beta-glucoside from Fritillaria pallidiflora Schrenk and quantitative determination by HPLC-evaporative light scattering detection
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AbstractThe extraction procedure and quantitative determination by HPLC-evaporative light scattering detection (ELSD) of the main bioactive components, namely, imperialine (1) and imperialine-3beta-glucoside (2), of bulbs of Fritillaria pallidiflora Schrenk have been investigated. The most efficient method for the simultaneous extraction of 1 and 2 involved pre-treatment of the bulb powder with ammonia, followed by reflux with dichloromethane:methanol at 90degreesC for 4 It. Simultaneous determination of non-chromophoric 1 and 2 by HPLC-ELSD employed a Kromasil(TM) C-18 Column eluted with acetonitrile: water: diethylamine. The assay was accurate and reproducible with an overall variation lower than 4% and a sample recovery higher than 98%. The methods described have been successfully used to evaluate the quality of three batches of the crude traditional Chinese medicinal herb derived from the bulbs of F. pallidiflora. Copyright (C) 2002 John Wiley Sons, Ltd.
All Author(s) ListLi P, Zeng LJ, Li SL, Lin G
Journal namePhytochemical Analysis
Volume Number13
Issue Number3
Pages158 - 161
LanguagesEnglish-United Kingdom
Keywordsextraction; Fritillaria pallidiflora; HPLC-ELSD; imperialine; imperialine-3 beta-glucoside; quantitative determination
Web of Science Subject CategoriesBiochemical Research Methods; BIOCHEMICAL RESEARCH METHODS; Biochemistry & Molecular Biology; Chemistry; Chemistry, Analytical; CHEMISTRY, ANALYTICAL; Plant Sciences; PLANT SCIENCES

Last updated on 2020-02-12 at 00:29