Cellular signaling mechanisms underlying pharmacological action of Bak Foong Pills on gastrointestinal secretion
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AbstractBak Foong Pills (BFP, also known as Bai Feng Wan) is an over-the-counter traditional Chinese medicine that has long been used for treating gynecological disorders and improving overall body functions, including gastrointestinal (GI) function. However, the cellular signaling mechanism underlying BFP action, especially on the Gl tract, has not been elucidated. In the present study, the human colonic epithelia cell line T-84 was used as a model to investigate the effect of BFP ethanol extract on ion transport in conjunction with the short-circuit current (/(sc)) technique. The results showed that the apical addition of BFP extract produced a concentration-dependent (10-1,000 mug/ml, EC50=120 mug/ml) increase in /sc. The maximal response was observed at 500 mug/ml with an increase in /(sc) of 24.4 +/- 2.3 muA/cm(2) and apical conductance. The BFP-induced /(sc) was not observed when extracellular Cl- was replaced or when treated with Bumetanide (100 muM), an inhibitor of the Na+-K+- 2Cl(-) cotransporter. The BFP-induced /(sc) was insensitive to the Na+ channel blocker, amiloride, but partially inhibited by the Cl- channel blocker, DIDS (100 muM), and completely blocked by DPC (2 mm) or glibenclamide (1 mm) with a significant reduction in the apical conductance. The BFP-induced /sc could be mimicked by forskolin (10 muM), but inhibited by a pretreatment of the cells with adenylate cyclase inhibitor, MDL-12330A (10 muM). Pretreatment with EGTA (5 mm) and thapsigargin (10 muM) decreased the BFP-induced /(sc) by 10%. These results demonstrated that BFP ethanol extract exerted a stimulatory effect on gastrointestinal Cl- secretion by predominantly activating adenylate cyclase and apical cAMP-dependent Cl- channels, with minor contributions from calcium-dependent Cl- channels. The effect of BFP may be explored to treat Gl disorders such as constipation.
All Author(s) ListZhu JX, Chan YM, Tsang LL, Chan LN, Zhou Q, Zhou CX, Chan HC
Journal nameJapanese Journal of Physiology
Volume Number52
Issue Number1
Pages129 - 134
LanguagesEnglish-United Kingdom
KeywordsBai Feng Wan; Bak Foong Pills; cAMP; Cl- secretion; T-84 cells
Web of Science Subject CategoriesPhysiology

Last updated on 2020-20-10 at 02:38