Correlation of vacuole morphology with stomatal lineage development by whole-cell electron tomography
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AbstractStomatal movement is essential for plants to optimize transpiration and therefore photosynthesis. Rapid changes in the stomatal aperture are accompanied by adjustment of vacuole volume and morphology in guard cells (GCs). In Arabidopsis (Arabidopsis thaliana) leaf epidermis, stomatal development undergoes a cell-fate transition including four stomatal lineage cells: meristemoid, guard mother cell, young GC, and GC. Little is known about the mechanism underlying vacuole dynamics and vacuole formation during stomatal development. Here, we utilized whole-cell electron tomography (ET) analysis to elucidate vacuole morphology, formation, and development in different stages of stomatal lineage cells at nanometer resolution. The whole-cell ET models demonstrated that large vacuoles were generated from small vacuole stepwise fusion/maturation along stomatal development stages. Further ET analyses verified the existence of swollen intraluminal vesicles inside distinct vacuoles at certain developmental stages of stomatal lineage cells, implying a role of multivesicular body fusion in stomatal vacuole formation. Collectively, our findings demonstrate a mechanism mediating vacuole formation in Arabidopsis stomatal development and may shed light on the role of vacuoles in stomatal movement.
Acceptance Date22/12/2021
All Author(s) ListWenhan Cao, Zhenping Li, Shuxian Huang, Yuwei Shi, Ying Zhu, Man Nga Lai, Pui Lok Lok, Xiangfeng Wang, Yong Cui, Liwen Jiang
Journal namePlant Physiology
Year2022
Month4
Volume Number188
Issue Number4
PublisherOxford University Press
Place of PublicationUSA
Pages2085 - 2100
ISSN0032-0889
eISSN1532-2548
LanguagesEnglish-United States

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