Stimulation of chloride secretion by baicalein in isolated rat distal colon
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AbstractThe effect of baicalein on mucosal ion transport in the rat distal colon was investigated in Ussing chambers. Mucosal addition of baicalein (1-100 muM) elicited a concentration-dependent short-circuit current (I-sc) response. The increase in I-sc was mainly due to Cl- secretion. The presence of mucosal indomethacin (10 muM) significantly reduced both the basal and subsequent baicalein-evoked I-sc responses. The baicalein-induced I-sc were inhibited by mucosal application of diphenylamine-2-carboxylic acid (100 muM) and glibenclamide (500 muM) and basolateral application of chromanol 293B (30 muM), a blocker of K(v)LQT1 channels and Ba2+ ions (5 mM). Treatment of the colonic mucosa with baicalein elicited a threefold increase in cAMP production. Pretreating the colonic mucosa with carbachol (100 muM, serosal) but not thapsigargin (1 muM, both sides) abolished the baicalein-induced I-sc. Addition of baicalein subsequent to forskolin induced a further increase in I-sc. These results indicate that the baicalein evoked Cl- secretion across rat colonic mucosa, possibly via a cAMP-dependent pathway. However, the action of baicalein cannot be solely explained by its cAMP-elevating effect. Baicalein may stimulate Cl- secretion via a cAMP-independent pathway or have a direct effect on cystic fibrosis transmembrane conductance regulator.
All Author(s) ListKo WH, Law VWY, Yip WCY, Yue GGL, Lau CW, Chen ZY, Huang Y
Journal nameAJP - Gastrointestinal and Liver Physiology
Volume Number282
Issue Number3
PagesG508 - G518
LanguagesEnglish-United Kingdom
Keywordscolonic mucosa; intracellular cyclic adenosine 5 '-monophosphate; isolated crypts; Ussing chambers
Web of Science Subject CategoriesGastroenterology & Hepatology; GASTROENTEROLOGY & HEPATOLOGY; Physiology; PHYSIOLOGY

Last updated on 2020-29-11 at 02:17