In vitro inhibition of head and neck cancer-cell growth by human recombinant interferon-alpha and 13-cis retinoic acid
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香港中文大學研究人員

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摘要Three nasopharyngeal carcinoma (NPC) cell lines (CNE-1 CNE-2 and NPC/HK-1), two squamous cell carcinoma (SCC) cell lines (T2/CUHK and PWH-S1) and six head and neck cancer specimens (NPC [n = 4], SCC tongue [n = 1] and a thyroid cancer [n = 1]) were incubated with interferon (IFN)-alpha (5 x 10(4) iu/mL) and/or 13-cis retinoic acid (13RA: 10(-5) mol/L) for two days at 37 degreesC. In vitro chemosensitivity was measured using MTT assay. Mild growth inhibition of the five cell lines by IFN-alpha ranged from 7.1% to 51.8% (mean: 18.5%), whereas with 13RA it was zero to 19.7% (mean: 7%). Greater inhibition (14.8-51.0%, mean: 31.8%) was achieved when the two drugs were used in combination. Growth inhibition of the six surgical specimens ranged from 6.9% to 21% (mean: 13.6%) with IFN-alpha, zero to 10.3% (mean: 6.0%) with 13RA; and 6.6-26.5% (mean: 17.7%) when the two agents were combined. Four of the 11 samples showed synergistic antitumour effect when IFN-alpha and 13RA were combined, and six showed subadditive effect. The results show that IFN-alpha and 13RA have a mild in vitro antitumour effect on head and neck cancer cells, and the drug synergistic effect demonstrated in this study suggests that the two agents should be used in combination in clinical application.
著者Lam PK, To EWH, Chan ESY, Liew CT, Lung IWH, King WK
期刊名稱British Journal of Biomedical Science
出版年份2001
月份1
日期1
卷號58
期次4
出版社ROYAL SOC MEDICINE PRESS LTD
頁次226 - 229
國際標準期刊號0967-4845
語言英式英語
關鍵詞head and neck neoplasms; interferon-alpha; isotretinoin; retinoids
Web of Science 學科類別Medical Laboratory Technology; MEDICAL LABORATORY TECHNOLOGY

上次更新時間 2021-27-02 於 01:25