Authentication of medicinal Dendrobium species by the internal transcribed spacer of ribosomal DNA
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AbstractHerba Dendrobii (Shihu) is a commonly used Chinese medicine derived from the stem of several orchid species belonging to the genus Dendrobium. it is rather expensive and adulteration is frequent. Proper authentication of the medicinal species is necessary to protect consumers and support conservation measures. DNA sequences of the internal transcribed spacer 2 (ITS 2) of 16 Dendrobium species were shown to be significantly different from one another by an average of 12.4% and from non-orchids and Pholidota (an adulterant of Shihu) by 29.8% and 18.8%, respectively, The intra-specific variation among the Dendrobium species studied was only about 1%, Therefore, ITS Z regions could be adopted as a molecular marker for differentiating medicinal Dendrobium species from one another and also from non-orchids and adulterants.
All Author(s) ListLau DTW, Shaw PC, Wang J, But PPH
Journal namePlanta Medica
Volume Number67
Issue Number5
Pages456 - 460
LanguagesEnglish-United Kingdom
KeywordsDendrobium; DNA sequence; endangered species; orchid; Orchidaceae
Web of Science Subject CategoriesChemistry, Medicinal; CHEMISTRY, MEDICINAL; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY; Plant Sciences; PLANT SCIENCES

Last updated on 2021-14-09 at 00:30