In situ model for studying potassium currents in various growth plate chondrocyte subpopulations
Publication in refereed journal


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摘要A new in situ model of partially digested growth plate cartilage suitable for patch clamp study of membrane currents of chondrocytes from various differentiation stages was developed. Thin sections of growth plate were enzyme digested to expose intact membranes of chondrocytes previously covered by extracellular matrix. This treatment dramatically increased the success rate of tight-seal formation from virtually 0% up to 40%. Whole-cell patch clamp recording revealed a delayed outward rectifying current as the major macroscopic current in chondrocytes of all differentiation stages. This current was sensitive to tetraethylammonium chloride and reversed polarity at a membrane potential close to the equilibrium potential of K+. Chondrocytes at resting stage expressed a much smaller K+ current than the proliferative and hypertrophic chondrocytes. When the current amplitudes were normalized for the cell membrane area, proliferative cells expressed a significantly higher outward current density. (C) 2001 Elsevier Science Inc. All rights reserved.
著者Lee KM, Ye GL, Yung WH, Leung KS, Leung PC
期刊名稱Life Sciences
詳細描述Life Sciences
出版年份2001
月份6
日期29
卷號69
期次6
出版社PERGAMON-ELSEVIER SCIENCE LTD
頁次721 - 728
國際標準期刊號0024-3205
電子國際標準期刊號1879-0631
語言英式英語
關鍵詞chondrocyte subpopulations; growth plate; potassium current; whole-cell recording
Web of Science 學科類別Medicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY; Research & Experimental Medicine

上次更新時間 2021-14-01 於 00:53