In situ model for studying potassium currents in various growth plate chondrocyte subpopulations
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AbstractA new in situ model of partially digested growth plate cartilage suitable for patch clamp study of membrane currents of chondrocytes from various differentiation stages was developed. Thin sections of growth plate were enzyme digested to expose intact membranes of chondrocytes previously covered by extracellular matrix. This treatment dramatically increased the success rate of tight-seal formation from virtually 0% up to 40%. Whole-cell patch clamp recording revealed a delayed outward rectifying current as the major macroscopic current in chondrocytes of all differentiation stages. This current was sensitive to tetraethylammonium chloride and reversed polarity at a membrane potential close to the equilibrium potential of K+. Chondrocytes at resting stage expressed a much smaller K+ current than the proliferative and hypertrophic chondrocytes. When the current amplitudes were normalized for the cell membrane area, proliferative cells expressed a significantly higher outward current density. (C) 2001 Elsevier Science Inc. All rights reserved.
All Author(s) ListLee KM, Ye GL, Yung WH, Leung KS, Leung PC
Journal nameLife Sciences
Detailed descriptionLife Sciences
Year2001
Month6
Day29
Volume Number69
Issue Number6
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Pages721 - 728
ISSN0024-3205
eISSN1879-0631
LanguagesEnglish-United Kingdom
Keywordschondrocyte subpopulations; growth plate; potassium current; whole-cell recording
Web of Science Subject CategoriesMedicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY; Research & Experimental Medicine

Last updated on 2020-08-08 at 02:18