Expression of cystic fibrosis transmembrane conductance regulator in rat efferent duct epithelium
Publication in refereed journal

香港中文大學研究人員

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摘要The expression of cystic fibrosis transmembrane conductance regulator (CFTR) was studied in rat efferent ducts. Under Whole-cell patch-clamp condition, efferent duct cells responded to intracellular cAMP with a rise in inward current. The cAMP-activated current exhibited a linear I-V relationship and time- and voltage-independent characteristics. The current was inhibited by the CI= channel blocker diphenylamine 2,2 ' -dicarboxylic acid (DPC) in a voltage-dependent manner and reversed at 24 +/- 0.5 mV, close to the equilibrium potential for CI- (30 mV), suggesting that the current was CI- selective. The cAMP-activated current displayed a permeability sequence of Br- > CI- > I-. Short-circuit current measurement in cultured rat efferent duct epithelia also revealed a cAMP-activated inward current inhibitable by DPC. These electrophysiological properties of the cAMP-activated CI- conductance in the efferent duct were consistent with those reported for CFTR. In support of the functional studies, reverse transcription polymerase chain reaction revealed the presence of CFTR message in cultured efferent duct epithelium. Immunohistochemical studies in intact rats also demonstrated CFTR protein at the apical membrane of the principal cells of efferent duct. CFTR may play a role in modulating fluid transport in the efferent duct.
著者Leung GPH, Gong XD, Cheung KH, Cheng-Chew SB, Wong PYD
期刊名稱Biology of Reproduction
出版年份2001
月份5
日期1
卷號64
期次5
出版社SOC STUDY REPRODUCTION
頁次1509 - 1515
國際標準期刊號0006-3363
電子國際標準期刊號1529-7268
語言英式英語
關鍵詞epididymis; male reproductive tract
Web of Science 學科類別Reproductive Biology; REPRODUCTIVE BIOLOGY

上次更新時間 2021-26-02 於 01:45