Characterization of nucleoside transport systems in cultured rat epididymal epithelium
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香港中文大學研究人員

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摘要The nucleoside transport systems in cultured epididymal epithelium were characterized and found to be similar between the proximal (caput and corpus) and distal (cauda) regions of the epididymis. Functional studies revealed that 70% of the total nucleoside uptake was Na+ dependent, while 30% was Na+ independent. The Na+-independent nucleoside transport was mediated by both the equilibrative nitrobenzylthioinosine (NBMPR)-sensitive system (40%) and the NBMPR-insensitive system (60%), which was supported by a biphasic dose response to NBMPR inhibition. The Na+-dependent [H-3] uridine uptake was selectively inhibited 80% by purine nucleosides, indicating that the purine nucleoside-selective N1 system is predominant. Since Na+-dependent [H-3] guanosine uptake was inhibited by thymidine by 20% and Na+-dependent [H-3] thymidine uptake was broadly inhibited by purine and pyrimidine nucleosides, this suggested the presence of the broadly selective N3 system accounting for 20% of Na+-dependent nucleoside uptake. Results of RT-PCR confirmed the presence of mRNA for equilibrative nucleoside transporter (ENT) 1, ENT2, and concentrative nucleoside transporter (CNT) 2 and the absence of CNT1. It is suggested that the nucleoside transporters in epididymis may be important for sperm maturation by regulating the extracellular concentration of adenosine in epididymal plasma.
著者Leung GPH, Ward JL, Wong PYD, Tse CM
期刊名稱American Journal of Physiology - Cell Physiology
出版年份2001
月份5
日期1
卷號280
期次5
出版社AMER PHYSIOLOGICAL SOC
頁次C1076 - C1082
國際標準期刊號0363-6143
電子國際標準期刊號1522-1563
語言英式英語
關鍵詞sperm maturation
Web of Science 學科類別Cell Biology; CELL BIOLOGY; Physiology; PHYSIOLOGY

上次更新時間 2021-26-02 於 01:44