Determination of the major isosteroidal alkaloids in bulbs of Fritillaria by high-performance liquid chromatography coupled with evaporative light scattering detection
Publication in refereed journal

香港中文大學研究人員

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摘要A new direct HPLC analytical method using evaporative light scattering detection coupled with a low-temperature adapter for the simultaneous determination of the major biologically active isosteroidal alkaloids in Bulbus Fritillariae, a commonly used antitussive traditional Chinese medicinal (TCM) herb, has been developed, The simultaneous separation of eight Fritillaria alkaloids was achieved on a reversed-phase C-8 column with an isocratic mobile phase system consisting of acetonitrile-methanol-water (66.5:3.5:30, v/v) containing 0.006% triethylamine. This method provides good reproducibility and sensitivity for the quantification of six major isosteroidal alkaloids, namely peimissine, verticine, verticinone, imperialine, isoverticine and ebeiedine in different Fritillaria species with overall intra- and inter-day precision and accuracy of less than 11% and higher than 90%, respectively. The assay was successfully utilized to quantify the major biologically active alkaloids in five Fritillaria species. The results demonstrate that this method is simple, selective, and suitable for the quality control of this commonly used antitussive TCM herb, Bulbus Fritillariae. (C) 2001 Elsevier Science B.V. All rights reserved.
著者Li SL, Lin G, Chan SW, Li P
期刊名稱Journal of Chromatography A
出版年份2001
月份2
日期16
卷號909
期次2
出版社ELSEVIER SCIENCE BV
頁次207 - 214
國際標準期刊號0021-9673
電子國際標準期刊號1873-3778
語言英式英語
關鍵詞Fritillaria spp.; isosteroidal alkaloids
Web of Science 學科類別Biochemical Research Methods; BIOCHEMICAL RESEARCH METHODS; Biochemistry & Molecular Biology; Chemistry; Chemistry, Analytical; CHEMISTRY, ANALYTICAL

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