Modulatory effect of protein kinase C activator on contractility of rat vas deferens
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AbstractThe modulatory effect of the protein kinase C activator was examined on contraction of rat isolated vas deferens induced by constrictive agonists, noradrenaline (NA), ATP, BaCl2 and high K+. Phorbol 12,13-diacetate (PDA, 1 mu mol/l) induced a transient extracellular Ca2+-dependent contraction while the inactive analogue, 4 alpha -phorbol (1 mu mol/l) had no effect. PDA significantly enhanced the peak amplitude of the contractile response to NA (0.110 mu mol/l), ATP (100 mu mol/l), Ba2+ (3 mmol/l) or high K+ (30 mmol/l). Staurosporine at 30 nmol/l reduced the enhancing effect of PDA on the agonist-induced contraction. NA (10 mu mol/l) produced a phasic contraction followed by a sustained contraction, while ATP induced monophasic contraction. Pretreatment with nifedipine (10 nmol/l) had no effect on the phasic contraction induced by NA, but it significantly reduced ATP- or high K+-induced contraction. Staurosporine (30 nmol/l) alone attenuated the peak contractile response induced by NA or ATP but not by Ba2+. NA produced a transient contraction in Ca2+-free Krebs solution, and PDA (1 mu mol/l) markedly enhanced this effect. These novel data indicate that activation of a protein kinase C-dependent mechanism not only affects contraction mediated by Ca2+ influx through voltage-sensitive Ca2+ channels, but also promotes intracellular Ca2+ release or intracellular Ca2+ mediated contractile mechanism in rat vas deferens. Copyright (C) 2001 S. Karger AG, Basel.
All Author(s) ListHuang Y, Pai RK, Lau CW, Chan FL, Chen ZY, Yao XQ
Journal namePharmacology
Volume Number62
Issue Number1
Pages2 - 9
LanguagesEnglish-United Kingdom
Keywordscontraction; phorbol ester; protein kinase C; smooth muscle; vas deferens, rat
Web of Science Subject CategoriesPharmacology & Pharmacy; PHARMACOLOGY & PHARMACY

Last updated on 2020-26-10 at 01:39