Programmed cell death is not involved in initiation of the gill cavity of Coprinus cinereus: A study using morphological mutants
Refereed conference paper presented and published in conference proceedings


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AbstractMushroom development results from a co-ordination of independent genetic and physiological subroutines. The nature of the subroutines involved and how and when they are invoked creates the spatial and temporal morphological pathway that is the taxonomic characteristic of a species. Morphogenesis can require the removal of tissue as well as tissue growth. The cell death responsible for this removal must be controlled in time and position as part of the differentiation process. This, called programmed cell death (PCD), could form a developmental subroutine able to sculpture tissue blocks from the hyphal mass which makes up the fruit body initial and primordium. PCD is highly organized in animals, though it occurs in plants, too. It is important to appreciate that the apoptotic cell death that occurs in vertebrates is a specialized form of PCD in which most of the process is kept inside an intact cell membrane to avoid leakage of antigens and consequent autoimmunity. Fungal PCD can, therefore, be very different from animal PCD because the immune reaction is not an issue. Indeed, fungal PCD appears to involve lysis of hyphal compartments. Recent work suggests that eel death is involved in formation of gill cavities in Agaricus bisporus. However, no sign has been found of cell disintegration/cell death during gill formation in the mushroom of Coprinus cinereus. Gill formation in this mushroom has been interpreted as featuring branches of determinate growth being organized into opposing palisade cell plates, forming an incipient fracture plane. This plane being opened out into a cavity when expansion of underlying tissue put tension across the 'fracture' and pulled the palisades apart. Morphological mutants have been used very effectively to dissect morphogenetic pathways in Coprinus cinereus. A hymenophoreless mutant is interesting in this context as the hymenophore bearing gills must arise within the-fruit body cap. So a mutant unable to make hymenophores enables us to determine whether programmed cell death is involved in defining the pathway and architecture of a hymenophore. We find that in hymenophoreless mutants of C. cinereus, cap expansion and sufficient consequential mechanical stress to cause cell disruption and create space definitely occur. However, no gills are formed. In contrast, the revoluta mutant (in which the cap does not enclose the stem) is able to make mature gills but no cell death is involved. In neither sort of mutants is open space a committing step for gill cavity formation. This leads us to conclude that the key is the patterning of hyphal tips into the palisades which become opposing hymenia, and not the formation of a primordial gill space. Thus, a gill organizer must be present to make girls in Coprinus.
All Author(s) ListChiu SW, Moore D
Name of Conference15th International Congress on the Science and Cultivation of Edible Fungi
Start Date of Conference15/05/2000
End Date of Conference19/05/2000
Place of ConferenceMAASTRICHT
Country/Region of ConferenceNetherlands
Year2000
Month1
Day1
PublisherA A BALKEMA PUBLISHERS
Pages115 - 119
ISBN90-5809-143-0
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesAgriculture; Agronomy; Mycology; Plant Sciences

Last updated on 2021-21-02 at 01:22