Hyperthermia and tumour necrosis factor-alpha induced apoptosis via mitochondrial damage
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摘要Hyperthermia is a potential anti-cancer regimen but the mode of action is far from clear. Based on the flow cytometric analysis with FITC-annexin V and propidium iodide, apoptosis was found to be the major form of cell death after the treatment with hyperthermia (43 degrees C, 3h) and/or recombinant murine tumour necrosis factor-alpha (TNF-alpha, 50ng/ml) in L929 cells. Since mitochondria are thought to play a key role in apoptosis, experiments were done to assess their role in the hyperthermia-mediated apoptosis. Our results indicate that hyperthermia was able to depolarize the mitochondrial membrane potential (Delta Psi m) and release cytochrome c to the cytoplasm, in a way very similar to the action of TNF-alpha. With the use of cyclosporin A to inhibit the Delta Psi m dissipation, the cytotoxicity mediated by hyperthermia or TNF-alpha was suppressed. Taken together, our results indicate that hyperthermia and TNF-alpha can induce apoptosis in L929 cells and the mitochondrial dysfunction plays a key role in the cell death process. (C) 2000 Elsevier Science Inc. All rights reserved.
著者Yuen WF, Fung KP, Lee CY, Choy YM, Kong SK, Ko S, Kwok TT
期刊名稱Life Sciences
出版年份2000
月份6
日期30
卷號67
期次6
出版社PERGAMON-ELSEVIER SCIENCE LTD
頁次725 - 732
國際標準期刊號0024-3205
電子國際標準期刊號1879-0631
語言英式英語
關鍵詞cytochrome c release; hyperthermia; mitochondria; mitochondrial potential; TNF
Web of Science 學科類別Medicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY; Research & Experimental Medicine

上次更新時間 2020-11-08 於 04:30