Hyperthermia and tumour necrosis factor-alpha induced apoptosis via mitochondrial damage
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AbstractHyperthermia is a potential anti-cancer regimen but the mode of action is far from clear. Based on the flow cytometric analysis with FITC-annexin V and propidium iodide, apoptosis was found to be the major form of cell death after the treatment with hyperthermia (43 degrees C, 3h) and/or recombinant murine tumour necrosis factor-alpha (TNF-alpha, 50ng/ml) in L929 cells. Since mitochondria are thought to play a key role in apoptosis, experiments were done to assess their role in the hyperthermia-mediated apoptosis. Our results indicate that hyperthermia was able to depolarize the mitochondrial membrane potential (Delta Psi m) and release cytochrome c to the cytoplasm, in a way very similar to the action of TNF-alpha. With the use of cyclosporin A to inhibit the Delta Psi m dissipation, the cytotoxicity mediated by hyperthermia or TNF-alpha was suppressed. Taken together, our results indicate that hyperthermia and TNF-alpha can induce apoptosis in L929 cells and the mitochondrial dysfunction plays a key role in the cell death process. (C) 2000 Elsevier Science Inc. All rights reserved.
All Author(s) ListYuen WF, Fung KP, Lee CY, Choy YM, Kong SK, Ko S, Kwok TT
Journal nameLife Sciences
Volume Number67
Issue Number6
Pages725 - 732
LanguagesEnglish-United Kingdom
Keywordscytochrome c release; hyperthermia; mitochondria; mitochondrial potential; TNF
Web of Science Subject CategoriesMedicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY; Research & Experimental Medicine

Last updated on 2020-10-07 at 04:22