Agonist-like activity of antibodies to angiotensin II receptor subtype 1 (ATI) from rats immunized with AT1 receptor peptide
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香港中文大學研究人員

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摘要In the present study, rats were immunized with angiotensin II receptor subtype 1 (AT1) receptor peptides for 3 months to see if the immunization produced specific anti-AT1 receptor antibodies and if continuous stimulation for 3 months affected blood pressure or induced morphological changes in the organs containing ATI receptors. Our results showed that there were constant high levels of circulating antibodies throughout the study period in all rats of the immunized group, but not in the control rats, and that there were almost no significant cross-reactions of antisera with AT2 receptor peptide and alpha 1 adrenoceptor peptide, except in four rats, which showed low cross-reactions with alpha 1 adrenoceptor and AT2 receptor peptides. When an affinity-purified anti-AT1 receptor antibody was used, it specifically displayed the AT1-stimulatory positive chronotropic effect and also localized AT1 receptors. However, in the immunized group, saturation binding of AT1 in homogenates from kidneys showed no difference either in maximal binding sites (Bmax) or in antagonist affinity (Kd). No difference in mRNA of AT1a was found in either kidney or heart, and no morphological changes in the organs were observed, as compared with the control group. Furthermore, immunization did not cause hypertension. In conclusion, the synthetic peptide corresponding to the second extra-cellular loop of the human AT1 receptor was able to produce highly specific and functionally active anti-AT1 receptor antibodies, but unable to induce pathological structural changes or hypertension.
著者Fu MLX, Leung PS, Wallukat G, Bergstrom G, Fu HM, Schulze W, Herlitz H
期刊名稱Blood Pressure
出版年份1999
月份1
日期1
卷號8
期次5-6
出版社SCANDINAVIAN UNIVERSITY PRESS
頁次317 - 324
國際標準期刊號0803-7051
語言英式英語
關鍵詞angiotensin II receptor; antibody; hypertension; immunofluorescence; peptide; telemetry
Web of Science 學科類別Cardiovascular System & Cardiology; Peripheral Vascular Disease; PERIPHERAL VASCULAR DISEASE

上次更新時間 2020-22-09 於 02:01