Protein-protein interaction of FHL2, a LIM domain protein preferentially expressed in human heart, with hCDC47
Publication in refereed journal


引用次數
替代計量分析
.

其它資訊
摘要In the yeast two-hybrid library screening, the heart-specific FHL2 protein was found to interact with hCDC47. In vitro interaction study between FHL2 protein and hCDC47 was demonstrated. From the results of domain studies by the yeast two-hybrid assay, the second and third LIM domains in conjunction with the first half LIM domain of FHL2 were identified to be important in binding with hCDC47. Besides, in Northern blot hybridization of human cancer cell lines, the highest FHL2 mRNA expression was detected in colorectal adenocarcinoma SW480 and HeLa cell S3. Our results imply that FHL2 protein may associate with cancer development and may act as a molecular adapter to form a multicomplex with hCDC47 in the nucleus, thus it plays an important role in the specification or maintenance of the terminal differentiated phenotype of heart muscle cells, (C) 2000 Wiley-Liss, Inc.
著者Chan KK, Tsui SKW, Ngai SM, Lee SMY, Kotaka M, Waye MMY, Lee CY, Fung KP
期刊名稱Journal of Cellular Biochemistry
出版年份2000
月份1
日期1
卷號76
期次3
出版社WILEY-LISS
頁次499 - 508
國際標準期刊號0730-2312
電子國際標準期刊號1097-4644
語言英式英語
關鍵詞heart cDNA; molecular adapter; yeast two-hybrid screen; zinc finger protein
Web of Science 學科類別Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Cell Biology; CELL BIOLOGY

上次更新時間 2020-04-08 於 02:34