Adrenaline upregulates monocyte L-selectin in vitro
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摘要Objective: Although surface adhesion molecules mediate leucocyte-endothelial interactions at sites of inflammation, relatively little is known of the factors which increase the expression of L-selectin in circulating leucocytes. The expression of leucocyte L-selectin increases during acute stress events such as injury and is temporally related to an early neuroendocrine response. This study investigates whether adrenaline increases the expression of L-selectin on monocytes, neutrophils and lymphocytes in vitro and whether these effects are mediated via beta-adrenoceptors. Methods: A total of 20 mi of blood was withdrawn from 28 healthy volunteers (21 males) with a mean age of 29 years (range 23-67 years). Adrenaline at physiological doses mimicking trauma (0-200 nmol/l) was added to whole blood prior to immunofluorescent staining and analysis by flow cytometry. Propranolol (50 mu l of 2 x 10(-5) M) was also added to separate tubes prior to incubation with adrenaline. Saline (40 mu l 0.9% solution) was used as a control. Expression is described firstly as percentage of cells expressing L-selectin and secondly as average intensity (mean channel fluorescence, mcf) per cell expressing CD62L. Summary measures were used to analyse the data. Results: A significant increase in both the percentage of monocytes expressing L-selectin and mean channel fluorescence of L-selectin was evident with adrenaline in vitro (P < 0.0001). Maximal increases occurred at 100 nmol/l adrenaline when a 9% increase in the percentage of monocytes expressing L-selectin and a 23% increase in mean channel fluorescence were observed. These effects were both blocked by propranolol (P < 0.0001). No significant differences were observed for neutrophils or lymphocytes except for a slight increase in the percent neutrophils expressing L-selectin, and a small decreasing trend in percent lymphocytes expressing L-selectin. Conclusions: Adrenaline upregulates the surface expression of L-selectin on monocytes in vitro, an effect which is partially mediated by beta-adrenoceptors. As monocytes initiate early aspects of the inflammatory response, these findings suggest that beta-blockade may have an inhibitory role for certain aspects of leucocyte trafficking. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
著者Rainer TH, Lam N, Cocks RA
期刊名稱Resuscitation
出版年份1999
月份12
日期1
卷號43
期次1
出版社ELSEVIER SCI IRELAND LTD
頁次47 - 55
國際標準期刊號0300-9572
語言英式英語
關鍵詞adhesion molecules; adrenaline; epinephrine; L-selectin; leucocytes; lymphocytes; monocytes; neutrophils
Web of Science 學科類別Critical Care Medicine; CRITICAL CARE MEDICINE; Emergency Medicine; EMERGENCY MEDICINE; General & Internal Medicine

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