Adrenaline upregulates monocyte L-selectin in vitro
Publication in refereed journal


摘要Objective: Although surface adhesion molecules mediate leucocyte-endothelial interactions at sites of inflammation, relatively little is known of the factors which increase the expression of L-selectin in circulating leucocytes. The expression of leucocyte L-selectin increases during acute stress events such as injury and is temporally related to an early neuroendocrine response. This study investigates whether adrenaline increases the expression of L-selectin on monocytes, neutrophils and lymphocytes in vitro and whether these effects are mediated via beta-adrenoceptors. Methods: A total of 20 mi of blood was withdrawn from 28 healthy volunteers (21 males) with a mean age of 29 years (range 23-67 years). Adrenaline at physiological doses mimicking trauma (0-200 nmol/l) was added to whole blood prior to immunofluorescent staining and analysis by flow cytometry. Propranolol (50 mu l of 2 x 10(-5) M) was also added to separate tubes prior to incubation with adrenaline. Saline (40 mu l 0.9% solution) was used as a control. Expression is described firstly as percentage of cells expressing L-selectin and secondly as average intensity (mean channel fluorescence, mcf) per cell expressing CD62L. Summary measures were used to analyse the data. Results: A significant increase in both the percentage of monocytes expressing L-selectin and mean channel fluorescence of L-selectin was evident with adrenaline in vitro (P < 0.0001). Maximal increases occurred at 100 nmol/l adrenaline when a 9% increase in the percentage of monocytes expressing L-selectin and a 23% increase in mean channel fluorescence were observed. These effects were both blocked by propranolol (P < 0.0001). No significant differences were observed for neutrophils or lymphocytes except for a slight increase in the percent neutrophils expressing L-selectin, and a small decreasing trend in percent lymphocytes expressing L-selectin. Conclusions: Adrenaline upregulates the surface expression of L-selectin on monocytes in vitro, an effect which is partially mediated by beta-adrenoceptors. As monocytes initiate early aspects of the inflammatory response, these findings suggest that beta-blockade may have an inhibitory role for certain aspects of leucocyte trafficking. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
著者Rainer TH, Lam N, Cocks RA
頁次47 - 55
關鍵詞adhesion molecules; adrenaline; epinephrine; L-selectin; leucocytes; lymphocytes; monocytes; neutrophils
Web of Science 學科類別Critical Care Medicine; CRITICAL CARE MEDICINE; Emergency Medicine; EMERGENCY MEDICINE; General & Internal Medicine

上次更新時間 2021-09-06 於 00:11