Angiotensinogen expression by rat epididymis: evidence for an intrinsic, angiotensin-generating system
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香港中文大學研究人員

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摘要Previous studies have suggested the presence of an intrinsic renin-angiotensin system (RAS) in the rat epididymis with functions in epididymal activity and sperm maturation. In the present study, the localization and expression of angiotensinogen, the component of the RAS which is indispensable for intracellular angiotensin generation, were investigated by immunochemistry, hybridization histochemistry and by reverse-transcriptase polymerase chain reaction (RT-PCR). Western blot analysis of protein from the epididymis confirmed the presence of angiotensinogen with the expected molecular mass of about 60 kDa, in agreement with results from other tissues. Immunocytochemistry showed the regional localization of immunoreactivity for angiotensinogen in the rat epididymis. In situ hybridization histochemistry further demonstrated the expression of angiotensinogen mRNA by the epididymal epithelium in a region-specific manner along the length of the rat epididymis. RT-PCR confirmed that the rat epididymis expresses angiotensinogen mRNA. On the other hand, mRNA of renin was not detected in the rat epididymis using Northern blot and RT-PCR analyses. The present study strongly supports the existence of an intrinsic, angiotensin-generating system based on locally formed angiotensinogen as a precursor for angiotensin production. This epididymal RAS may have paracrine or autocrine roles in mediating the epididymal and sperm functions. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
著者Leung PS, Wong TP, Sernia C
期刊名稱Molecular and Cellular Endocrinology
出版年份1999
月份9
日期10
卷號155
期次1-2
出版社ELSEVIER SCI IRELAND LTD
頁次115 - 122
國際標準期刊號0303-7207
語言英式英語
關鍵詞angiotensinogen; epididymis; renin; renin-angiotensin system
Web of Science 學科類別Cell Biology; CELL BIOLOGY; Endocrinology & Metabolism; ENDOCRINOLOGY & METABOLISM

上次更新時間 2020-25-09 於 02:28